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Bedops for extracting fasta sequences from bed file
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chudar:
Hi,
I am new to bedops and I have bed file like following
--- Code: ---bed_file
scaffold123 56 120 transcript_A
scaffold123 175 234 transcript_A
--- End code ---
I would like to extract the fasta sequence from the from the respective scaffold in genome.fasta with transcript header. Using bedtools this can be acheived by
--- Code: --- bedtools getfasta -name fi genome.fasta -bed bed_file
--- End code ---
Is it possible to achieve the same output using bedops. Kindly guide me.
AlexReynolds:
BEDOPS works with BED files and leaves work on FASTA files to other toolkits.
If you're looking for an alternative to bedtools, you might investigate samtools faidx.
Some examples here:
• https://www.biostars.org/p/183629/#183633
• https://www.biostars.org/p/98725/#98733
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